Marco Di Vaio's goal in the 28th minute was the difference between the two sides at the Renato Dall' Ara stadium.
The result was Bologna's first league win since October 30 and lifted Stefano Pioli's men four points clear of the relegation zone.
Siena, meanwhile, have dropped to 14th place, level on 14 points with Bologna.
Both teams went into the game having failed to win in their last three league encounters.
It was Siena that looked the most dangerous side in the opening stages and almost went ahead in the 25th minute.
Marcelo Larrondo got behind Gaetano D'Agostino's cross and his powerful header was saved by Bologna goalkeeper Jean Francois Gillet.
Bologna took the lead soon after Alessandro Diamanti fed Di Vaio inside the area and his right-footed strike from 10 yards beat Siena goalkeeper Zeljko Brkic.
Diamanti almost doubled his team's advantage after the half-hour mark but his left-footed strike from the heart of the area was kept out by Brkic.
Siena almost equalised before half-time when Alessandro Gazzi's header from D'Agostino's cross went inches wide of the far post.
Bologna continued to push forward after the re-start and were unlucky not to extend their lead before the hour mark.
Diamanti's powerful free-kick beat Brkic but struck the crossbar.
Siena had a great chance to equalise with 15 minutes remaining but Emanuele Calaio headed the ball just wide from Reginaldo's dangerous cross.
Bologna defended deeply and held on for victory as Siena's winless run on the road this season continued.
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